Human 25-hydroxyvitamin D(25-(OH)D) ELISA Kit
HumanThe kit uses a double-antibody sandwich enzyme-linked immunosorbent
assay (ELISA) to assay the level of Human 25-hydroxyvitamin D(25-(OH)D)
in samples. Add 25-hydroxyvitamin D(25-(OH)D) to monoclonal antibody
Enzyme well which is pre-coated with Human 25-hydroxyvitamin D(25-(OH)D)
monoclonal antibody, incubation; then, add 25-hydroxyvitamin D(25-(OH)D)
antibodies labeled with biotin, and combined with Streptavidin-HRP
to form immune complex; then carry out incubation and washing again to
remove the uncombined enzyme. Then add Chromogen Solution A, B,
the color of the liquid changes into the blue, And at the effect of acid,
the color finally becomes yellow. The chroma of color and the concenthumanion
of the Human Substance 25-hydroxyvitamin D(25-(OH)D) of sample were
positively correlated.
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