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Human 25-hydroxyvitamin D(25-(OH)D) ELISA Kit

Human
Human 25-hydroxyvitamin D(25-(OH)D) ELISA Kit
Human 25-hydroxyvitamin D(25-(OH)D) ELISA Kit

The kit uses a double-antibody sandwich enzyme-linked immunosorbent 

assay (ELISA) to assay the level of Human 25-hydroxyvitamin D(25-(OH)D) 

in samples. Add 25-hydroxyvitamin D(25-(OH)D) to monoclonal antibody 

Enzyme well which is pre-coated with Human 25-hydroxyvitamin D(25-(OH)D)

monoclonal antibody, incubation; then, add 25-hydroxyvitamin D(25-(OH)D)

antibodies labeled with biotin, and combined with Streptavidin-HRP 

to form immune complex; then carry out incubation and washing again to 

remove the uncombined enzyme. Then add Chromogen Solution A, B, 

the color of the liquid changes into the blue, And at the effect of acid, 

the color finally becomes yellow. The chroma of color and the concenthumanion 

of the Human Substance 25-hydroxyvitamin D(25-(OH)D) of sample were 

positively correlated.

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