Human ADH(VP or AVP) ELISA Kit
HumanThe kit assay Human ADH/VP/AVP level in the sample, use Purified Human ADH/VP/AVP to coat microtiter plate wells,
make solid-phase antibody, then add ADH/VP/AVP to wells, Combine ADH/VP/AVP antibody with HRP labeled to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution,TMB substrate
becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and
the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ADH/VP/AVP
in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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