Human Thiamine ELISA Kit
HumanFor the quantitative level of Human Thiamine level in the sample, adopt Purified Human Thiamine antibody to coat microtiter plate wells,
make solid-phase antibody, then Pipette Thiamine to wells, Combined Thiamine antibody which With labeled HRP to form antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed,
reaction is terminated by the addition of a solution and the color change is measured at a wavelength of 450 nm.
The concentration of PAG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product No.:
Technical support ,pls contact gs001@glorybios.com