Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase(ADI1) ELISA Kit
HumanThe kit is for the quantitative level of ADI1 in the sample, adopt purified human ADI1 to coat microtiter plate,
make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to ADI1,
then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate
becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is
terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm.
The concentration of ADI1 in the samples is then determined by comparing the O.D. of the samples to the
standard curve.
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