DETECTION RANGE
Detection range of the kit is: 5ng/L -100ng/L
The kit is for the quantitative level of LAM in the sample, adopt purified human LAM to
coat microtiter plate, make solid-phase antibody, then add samples or standards to
wells with a labeled antibody specific to LAM, then add labeled HRP to the well. After
washing completely, add TMB substrate solution, TMB substrate becomes blue
color in wells that contains antibody - antigen - enzyme-antibody complex, reaction
is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of LAM in the samples is then determined by
comparing the O.D. of the samples to the standard curve.