Human Platelet Factor 4 (PF4) ELISA Kit
HumanThe kit is for the quantitative level of PF4 in the sample, adopt purified Human PF4 to coat microtiter plate,
make solid-phase antibody, then add PF4 to wells, Combine PF4 antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution,
TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a
stop solution and the color change is measured at a wavelength of 450 nm. The concentration of PF4 in
the samples is then determined by comparing the O.D. of the samples to the standard curve.
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