Human Carbohydrate antigen19-9 (CA19-9) ELISA Kit
HumanThe kit is for the quantitative level of CA19-9 in the sample, adopt purified Human CA19-9 to coat microtiter
plate, make solid-phase antibody, then add CA19-9 to wells, Combine CA19-9 antibody with labeled HRP
to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution,
TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop
solution and the color change is measured at a wavelength of 450 nm. The concentration of CA19-9 in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
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