Search，Order，Antibody,ELISA Kit & Protein

The kit is for the quantitative level of CREB in the sample, adopt purified Rat CREB to coat microtiter plate, make solid-phase antibody, then add CREB to wells, Combine CREB antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. 

The concentration of CREB in the samples is then determined by comparing the O.D of the samples to the standard curve.