Human A60 IgG ELISA Kit
HumanThe kit is for the quantitative level of A60 IgG in the sample, adopt purified Human A60 IgG to coat microtiter
plate, make solid-phase antibody, then add A60 IgG to wells, Combine A60 IgG antibody with labeled HRP to
form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB
substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop
solution and the color change is measured at a wavelength of 450 nm. The concentration of A60 IgG in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
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