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11086Human Super Oxidase Dimutase (SOD) ELISA Kit.pdf

The kit assay Human SOD level in the sample, use Purified Human SOD to coat microtiter plate wells, make solid-phase antibody, then add SOD to wells, Combine SOD antibody with HRP labeled to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution,TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SOD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

DETECTION RANGE

Detection range of the kit is: 3mU/L - 160mU/L