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Human mammaglobin ELISA Kit

Human

The kit is for the quantitative level of mammaglobin in the sample, adopt purified human mammaglobin to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to mammaglobin, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of mammaglobin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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