Search，Order，Antibody,ELISA Kit & Protein

Introduction

l For the quantitative determination of CHE concentrations. 

l Detection of species: Human

l Detection medium: serum, plasma, tissue homogenates, cell culture supernates

Principle of test

The kit is for the quantitative level of CHE in the sample, adopt purified Human CHE to coat microtiter plate, make solid-phase antibody, then add CHE to wells, Combine CHE antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of CHE in the samples is then determined by comparing the O.D. of the samples to the standard curve. 

DETECTION RANGE

Detection range of the kit is: 15nmol/L-480pg/mL