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Introduction

l For the quantitative determination of IL-1R concentrations. 

l Detection of species: Human

l Detection medium: serum, plasma, tissue homogenates, cell culture supernates

Principle of test

The kit is for the quantitative level of IL-1R in the sample, adopt purified Human IL-1R to coat microtiter plate, make solid-phase antibody, then add IL-1R to wells, Combine IL-1R antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of IL-1R in the samples is then determined by comparing the O.D. of the samples to the standard curve. 

DETECTION RANGE

Detection range of the kit is: 25pg/mL -850pg/mL