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The kit is for the quantitative level of ALT in the sample, adopt purified Human ALT to coat microtiter plate, make solid-phase antibody, then add ALT to wells, Combine ALT antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of ALT in the samples is then determined by comparing the O.D. of the samples to the standard curve. 

DETECTION RANGE
Detection range of the kit is:1ng/ml-28ng/ml