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l  For the quantitative determination of Mouse IL-1αconcentrations.

l  Detection of species: Mouse

l  Detection medium: serum, plasma, tissue homogenates, cell culture supernates.

Principle of test

The kit is for the quantitative level of IL-1α in the sample, adopt purified Mouse IL-1α to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to IL-1α, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of IL-1α in the samples is then determined by comparing the O.D. of the samples to the standard curve.