Search，Order，Antibody,ELISA Kit & Protein

PRINCIPLE OF TEST
The kit is for the quantitative level of E-cad in the sample, adopt purified Mouse E-cad to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to E-cad, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of E-cad in the samples is then determined by comparing the O.D. of the samples to the standard curve.
DETECTION RANGE
Detection range of the kit is: 3ng/L -150ng/L