Search，Order，Antibody,ELISA Kit & Protein

Purpose
This kit allows for the determination of ABCG2 concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human ABCG2 level in the sample，use Purified Human ABCG2 antibody to coat microtiter plate wells, make solid-phase antibody, then add ABCG2 to wells, Combined ABCG2 which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ABCG2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.