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 For the quantitative determination of Rat Caspase-7 concentrations.

Detection of species: Rat

 Detection medium: serum, plasma, tissue homogenates, cell culture supernates

      The kit is for the quantitative level of Caspase-7 in the sample, adopt purified Rat Caspase-7 to coat microtiter plate, make solid-phase antibody, then add Caspase-7 to wells, Combine Caspase-7 antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of Caspase-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.