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l   For the quantitative determination of human SHBG concentrations.

l   Detection of species: Human

l   Detection medium: serum, cell culture supernatant, and other biological fluids

The kit is for the quantitative level of SHBG in the sample, adopt purified Human SHBGto coat microtiter plate, make solid-phase antibody, then add SHBG to wells, Combine SHBG antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of SHBG in the samples is then determined by comparing the O.D. of the samples to the standard curve.