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Chicken Salmonella IgA ELISA Kit

Chicken
Chicken Salmonella IgA ELISA Kit
Chicken Salmonella IgA ELISA Kit

This kit allows for the determination of Salmonella IgAexpression in Chicken serum, and other biological fluids.

Principle of test

The kit is for the qualitative determination of Salmonella IgA in the sample, adopt purified antigen to coat microtiter plate, make solid-phase antigen, then pipette samples to the wells, with anti- Salmonella IgA conjugated Horseradish Peroxidase (HRP). Wash and remove non-combinative antibody and other components. Antibodies specific for the antigen will bind to the pre-coated antigen. After washing completely, add TMB substrate solution and color develops according to the amount of Salmonella IgA. Reaction is terminated by the addition of a stop solution and the intensity of the color is measured at a wavelength of 450 nm. Compared with the CUTOFF value to judge if Salmonella IgAexists in the sample or not

Composition of the kit

Reagent

Quantity

Microelisa Stripplate

12well×8strips

Positive control

1×0.5ml

Negative control

1×0.5ml

HRP-Conjugate Reagent

1×6ml

Sample Diluent

1×6ml

Chromogen Solution A

1×6ml

Chromogen Solution B

1×6ml

Stop Solution

1×6ml

Wash Solution 30×

1×20ml

User manual

1

Adhesive Strip

2

Sealed bag

1


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Product No.:A0903

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