Search，Order，Antibody,ELISA Kit & Protein

1. Purpose
This kit is Aflatoxin Quantitative detection of residues in feed, fish, shrimp,
tissue, egg, honey, milk, serum and urine sample.
2. Principle
This kit uses a direct competitive ELISA method in microplate coated with
coupled antigen, adding standard or samples, free and pore strips Pre-coated
conjugated antigen compete against antibody enzyme marker color with TMB substrate
stop solution added after the color from blue to yellow, with a microplate reader
detection wavelength at 4 5 0 nm, the sample absorbance value and content is
inversely proportional to the sample through the standard curve content.
3. Kit composed
( 1)Pre-coated coupled antigen removable microtiter plates:1 block (12 wells×8 strips)
(2)Standard:6 bottles(1ml/vial),contents were:0 ng/ml, 0.2 ng/ml,0.6 ng/ml, 1.8
ng/ml,5.4 ng/ml, 16.2 ng/ml.
Anti- AFT- B1 antibody conjugate: 1 bottle (6ml)
(4) Color liquid A: 1 bottle (6ml)
(5) Color liquid B: 1 bottle (6ml)
(6) Stop Solution: 1 bottle (6ml), 2M sulfuric acid.
(7) Sample Diluent: 1 bottle ( 10 × , 6ml), used for sample dilution.
(8) washing liquid concentrate: 1 bottle (20 × , 20ml), for the washer
(9) A description
4. The material needs without providing
(1) Equipment
1) Wavelength 450nm microplate reader.
2) Mill.
3) Graduated cylinder.
4) Oscillator.
5) Funnel.
6) Whatman No 1 filter paper or equivalent.
7) Pipette.